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cholesterol fluorometric assay kit 10007640  (Cayman Chemical)

 
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    Cayman Chemical cholesterol fluorometric assay kit 10007640
    Cholesterol Fluorometric Assay Kit 10007640, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cholesterol fluorometric assay kit 10007640/product/Cayman Chemical
    Average 90 stars, based on 1 article reviews
    cholesterol fluorometric assay kit 10007640 - by Bioz Stars, 2026-03
    90/100 stars

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    HBV-miR-3 expression leads to hepatocyte <t>cholesterol</t> build-up. A: Experimental design of the <t>fluorometric</t> cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.
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    HBV-miR-3 expression leads to hepatocyte <t>cholesterol</t> build-up. A: Experimental design of the <t>fluorometric</t> cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.
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    https://www.bioz.com/result/cholesterol fluorometric assay kit 10007640/product/Cayman Chemical
    Average 90 stars, based on 1 article reviews
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    HBV-miR-3 expression leads to hepatocyte <t>cholesterol</t> build-up. A: Experimental design of the <t>fluorometric</t> cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.
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    HBV-miR-3 expression leads to hepatocyte <t>cholesterol</t> build-up. A: Experimental design of the <t>fluorometric</t> cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.
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    HBV-miR-3 expression leads to hepatocyte <t>cholesterol</t> build-up. A: Experimental design of the <t>fluorometric</t> cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.
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    HBV-miR-3 expression leads to hepatocyte <t>cholesterol</t> build-up. A: Experimental design of the <t>fluorometric</t> cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.
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    Average 90 stars, based on 1 article reviews
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    assay kits for the fluorometric measurement of cholesterol  (Cayman Chemical)
    90
    Cayman Chemical assay kits for the fluorometric measurement of cholesterol
    HBV-miR-3 expression leads to hepatocyte <t>cholesterol</t> build-up. A: Experimental design of the <t>fluorometric</t> cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.
    Assay Kits For The Fluorometric Measurement Of Cholesterol, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/assay kits for the fluorometric measurement of cholesterol/product/Cayman Chemical
    Average 90 stars, based on 1 article reviews
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    HBV-miR-3 expression leads to hepatocyte cholesterol build-up. A: Experimental design of the fluorometric cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.

    Journal: Journal of Lipid Research

    Article Title: HBV-miR-3 induces hepatic cholesterol accumulation by targeting ABCA1 : Evidence for potential benefits of statin usage

    doi: 10.1016/j.jlr.2025.100866

    Figure Lengend Snippet: HBV-miR-3 expression leads to hepatocyte cholesterol build-up. A: Experimental design of the fluorometric cholesterol estimation assay performed for evaluating the expected increase in intracellular cholesterol post HBV-miR-3 expression in hepatocytes. B: Cellular cholesterol levels in cells (Huh7, HepG2) 48h after transfection with pHBV-miR-3, pcDNA3.1+, HBV 1.3x and microRNA-Scramble control (miR-SC). The cholesterol levels were normalised to respective total protein content. Bar graphs represent mean (±SD) of three independent experiments. C: Left panel: Hepatic lipid droplet accumulation visualised by BODIPY 493/503 staining in cells (Huh7, HepG2) 48 h post transfection with pHBV-miR-3 and pcDNA3.1+. The cells were counterstained with nuclear stain DAPI. Scale bar: 20 μM. Right panel: Quantitative analysis of BODIPY 493/503 fluorescence intensity was quantified in each micrograph and expressed as the mean ± standard errors (n = 6) in arbitrary units (a.u). ∗ P ≤ 0.05.

    Article Snippet: The second aliquot was subjected to lipid extraction as described earlier ( ) followed by estimation of total cellular cholesterol content using the Amplex red cholesterol fluorometric assay kit (ThermoFisher Scientific).

    Techniques: Expressing, Transfection, Control, Staining, Fluorescence